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NBT:Sequencing of Allotetraploid Cotton

发布人:道斯夫生物
时间:2015-04-27 11:17:29
Sequencing of Allotetraploid Cotton (Gossypium hirsutum L. acc. TM-1) Provides a Resource for Fiber Improvement
 
Zhang T, Hu Y, Jiang W, et al.
 
Abstract
 
       Upland cotton is a model for polyploid crop domestication and transgenic improvement. Here we sequenced the allotetraploid Gossypium hirsutum L. acc. TM-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end sequences and genotype-by-sequencing genetic maps. We assembled and annotated 32,032 A-subgenome genes and 34,402 D-subgenome genes. Structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the A subgenome than in the D subgenome, suggesting asymmetric evolution. However, no genome-wide expression dominance was found between the subgenomes. Genomic signatures of selection and domestication are associated with positively selected genes (PSGs) for fiber improvement in the A subgenome and for stress tolerance in the D subgenome. This draft genome sequence provides a resource for engineering superior cotton lines.
 
Nat Biotechnol. 2015 Apr 20. doi: 10.1038/nbt.3207.
 
http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3207.html

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